The Qizhi Weitong Granules Alleviate Functional Dyspepsia-like Gastric Hypersensitivity in Rats by Inhibiting Inflammatory Cytokines and Strengthening Duodenal Mucosal Barrier
DOI:
https://doi.org/10.54844/gmiw.2023.0532Keywords:
Qizhi Weitong granules, functional dyspepsia, inflammatory cytokine, duodenal mucosal barrier, visceral hypersensitivityAbstract
Background: Qizhi Weitong (QZWT) granules are a compound preparation of Chinese medicine, which are approved by the
China Food and Drug Administration for the treatment of functional dyspepsia (FD) and have been used in clinical practice for
decades. The objective of this study is to evaluate whether QZWT granules can alleviate gastric hypersensitivity by improving
low-grade inflammation and the mucosal barrier in the duodenum in a functional dyspepsia-like (FD-like) model in rats.
Methods: FD-like gastric hypersensitivity in adulthood was induced by iodoacetamide (IA) in neonatal rats. Thirty-two
Sprague-Dawley (SD) rats were randomly classified into the control, model, QZWT, and Mosapride groups. Further, 0.2 mL
of 0.1% iodoacetamide and 2.0% sucrose administrated by oral gavage was used to establish the FD-like rats model (once
a day, for six consecutive days). Electromyography (EMG) was used to evaluate the visceral motor response to gastric
dilation. After treatment, the fluorescein isothiocyanate-glucan (FITC-glucan) test was performed to evaluate duodenal
permeability; enzyme-linked immunosorbent assay (ELISA) and quantitative polymerase chain reaction (qPCR) were used to
evaluate the pro-inflammatory cytokines for duodenal epithelial cells and the tight junction (TJ) proteins of the duodenal
mucosa. Results: The EMG score was elevated in the model group compared to the control group, but it decreased after
QZWT intervention; no changes were observed between the Mosapride and model groups. Compared with the control
group, rats in the gastric hypersensitivity model group showed a higher expression of interleukin-6 (IL-6) and tumor necrosis
factor-α (TNF-α) and lower expression of zona occludens protein 1 (ZO-1), occludin, and desmosome (DSG2) of junction
proteins (P < 0.05); while in the QZWT group, the expression of IL-6 and TNF-α was reduced (P < 0.01), and the expression
of ZO-1, occludin, and DSG2 was increased compared to the model group (P < 0.05). Conclusion: QZWT granules
alleviate visceral hypersensitivity in an FD-like model in rats by inhibiting inflammatory cytokines IL-6 and TNF-α and
regulating junction proteins.
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